A Human Primary in vitro Model of the Glomerular Filtration Barrier for Disease Modelling
Time: 3:00 pm
day: Day 1 Track 1 PM
Details:
- Primary podocyte model maintains high marker expression, including Nephrin, Podocin, WT1, and CD2AP
- TNF sensitivity leads to de-differentiation and decreased barrier integrity, with no effect on 70 kDa FITC-dextran permeability or cell viability
- Co-culture with Glomerular Microvascular Endothelial Cells presents an in vivo-like environment for disease modelling
